Interestingly, the growth of xenografted tumours, the mouse survival rate, and melanoma cell proliferation were not affected in the absence of host Lrg1. Considering the large amount of Lrg1 produced by B16F10 cells, we further validated the role of Lrg1 on melanoma cell growth using an in vitro cell-based assay, which again showed no impact of Lrg1 on melanoma cell viability and proliferation. Here, LRG1 is linked to melanoma.