AGO4 and infection: Indeed, previously published RNA-seq data of IPO323 obtained at 4, 11, 13 and 20 days post inoculation (dpi) [65] indicates a higher expression of Ago1 compared to Ago2, Ago3, Ago4 and Dcl. However, for all five genes, there are no significant changes in gene expression levels during the time course of host infection (Figure S6D).