To test the role of hydroxy groups of 2 in its interaction with hNKA, the computer simulated docking profiles for digitoxin (1), digoxin (2), or the acetylated derivatives of 2, including (+)-12,4′c-di-O-acetyldigoxin (3), (+)-12,3′c,4′c-tri-O-acetyldigoxin (4), and (+)-12,3′a,3′b,3′c,4′c-penta-O-acetyldigoxin (5) (Figure 2A), and hNKA were investigated; and the docking scores and cytotoxicity toward human cancer cells are presented in Table 1. Here, KCNV1 is linked to cancer.