Sera from all six pythiosis subjects (HuP1–3 and HsP1–3) and the PiCFA-immunized rabbit (RbCFA) reacted with multiple proteins of PiCFA (15–120 kDa), including some prominent bands (15, 25, 37, 40–50, 55, 60, 65, 70, 75, and 120 kDa) (Figure 3A). This evidence concerns the gene SYPL1 and Pythiosis.