SYPL1 and Pythiosis: The RbCFA, pythiosis (HyP1–3 and HsP1–3), and control (HuC1 and HsC1–5) serum samples showed no WB signal against most B. ranarum proteins, except three faint bands: 20 kDa (for HsP1); 25 kDa (for RbCFA, HsP2, HsP3, HsC1, HsC3, and HsC5); and 75 kDa (for HsC4) (Figure 3B).