We previously used a combinatorial approach (ganglioside quantification by MALDI-TOF, RT-qPCR data for glycosyltransferase/CASD1 gene expression, and the use of the “Ganglio-sphingolipid metabolism” pathway from WikiPathways) to perceive the relationships between gangliosides expressed and glycosyltransferase (GTs) gene expression between two breast cancer cell lines. The gene discussed is CASD1; the disease is breast cancer.