CD19 and B-cell chronic lymphocytic leukemia: EGFRt/19-28z/4-1BBL CAR-T cells comprise T cell enrichment, activation, and genetic modification by a retroviral vector encoding a CD19-targeted CAR, the co-stimulatory ligand 4-1BBL, and the EGFRt safety system (EGFRt/19-28z/4-1BBL) from peripheral blood of subjects that have relapsed or refractory chronic lymphocytic leukemia (CLL).