CD44 and EpCAM markers, suitable for staining in flow cytometry, enabled the establishment of a gating strategy aiming at identifying not only the putative CSC (EpCAMhigh/CD44high) and the non-stem cancer cell (EpCAMlow/−/CD44low/−) populations, but also putative intermediate cell populations (EpCAMhigh/CD44low/− and EpCAMlow/−/CD44high populations) (Figure 3A). This evidence concerns the gene CD44 and cancer.