To validate the requirement of ALKBH5 in the promotion/transformation of AML cells, Krivtsov et al., 2006 used MLL-AF9 (MA9)-induced leukemogenesis model (where MLL-rearranged fusion protein alone is sufficient to transform normal HSPCs into leukemic cells coupled with ALKBH5 knockout (ALKBH5KO) model [91]. This evidence concerns the gene MLLT3 and acute myeloid leukemia.