Here, to determine whether S100B expression is elevated in LCs from vitiligo, we analyzed sections from eight lesional skin samples of non-segmental vitiligo in a progressive state, ten non-segmental vitiligo in a stable state, and five healthy controls using four-color fluorescent immunohistochemistry to stain for melanocytes (white), LCs (green), S100B (red), and nuclei (blue) (Figure 4a). This evidence concerns the gene S100B and vitiligo.