In brief, our study quantifies the global m6A level in HNSCC cell lines and cancerous and paired-matched histopathologically unchanged tissues and correlates its content with expression of selected RNA methylation methyltransferase (METTL3), demethylase (FTO), and m6A binding proteins (YTHDF2, YTHDC2). Here, YTHDC2 is linked to head and neck squamous cell carcinoma.