In brief, our study quantifies the global m6A level in HNSCC cell lines and cancerous and paired-matched histopathologically unchanged tissues and correlates its content with expression of selected RNA methylation methyltransferase (METTL3), demethylase (FTO), and m6A binding proteins (YTHDF2, YTHDC2). The gene discussed is YTHDF2; the disease is head and neck squamous cell carcinoma.