Methylation analysis of CRFR2 using the same dataset revealed that three CpGs (cg04863452, cg15615793, cg04923928) and one CpG (cg18351440, located in the N shelf) were hypermethylated and hypomethylated, respectively, in CRC ccfDNAs compared to their normal counterparts. This evidence concerns the gene CRHR2 and colorectal carcinoma.