Micro-Raman spectroscopy has been implemented to observe and check the formation processes of the h-k-SOI-NW chip surface with high-k coating (h-k-SOI-NW chip), which was later used for the detection of oDNA, i.e., a synthetic analogue of circ-SHKBP1, which correlates with the development of human glioma [16]. Here, SHKBP1 is linked to central nervous system cancer.