Moreover, in either A549 cells treated with H2O2 or M17 neuroblastoma cells treated with paraquat, the recovery of DJ-1 from an oxidized to reduced DJ-1 form after removal of stimuli took 6–24 h; recovery was completely blocked in the presence of cycloheximide (CHX, a de novo protein synthesis inhibitor), demonstrating new protein synthesis is required for recovery [27,45]. This evidence concerns the gene PARK7 and neuroblastoma.