In this study, we expanded our previous research by examining the mechanism of IL-6 induction of angiogenic factors via proliferation-related JAK1/2, PI3K, and mTOR signaling in JAK2V617F positive HEL cells and mononuclear cells (MNC contains lymphocytes (T cells) and monocytes) of patients with MPN. This evidence concerns the gene MTOR and myeloproliferative disorder.