Next, to decipher the underlying mechanism of autophagy and apoptosis crosstalk, we performed a Co-Immunoprecipitation (Co-IP) assay and examined the effect of Nic on the physical binding of Beclin-1 with BCL2 in PC cell lines treated with Nic for various time point (24 h & 48 h). The gene discussed is BECN1; the disease is pachyonychia congenita.