Additionally, we also observed the downregulation of mesenchymal markers, i.e., N-cadherin, no effect on the expression of epithelial marker E-cadherin, and claudin-1 in COLO 357 and SW1990 cells treated with Nic (5 μM and 10 μM) (Supplementary Figure S2B) leading to inhibition of migration potential of PC cells. This evidence concerns the gene CLDN1 and pachyonychia congenita.