As expected, PD1 levels in spleen samples of transplanted WT mice increased significantly in the CD4 and CD8 T cell compartment upon tumor injection compared to untransplanted mice (CD8 Tx: mean 42.3%, SD 18.1%, n = 12; CD8 noTx: mean 4.2%, SD 2.4%, n = 3, p = 0.003; CD4 Tx: mean 45.5%, SD 16.5%, n = 12; CD4 noTx: mean 20.3%, SD 9.2%, n = 3, p = 0.02) (Figure 3B,C). This evidence concerns the gene CD4 and neoplasm.