PKM and cancer: By using an inducible cell line in which the DIO2 gene can be overexpressed upon doxycycline (DOX, 2 μg/mL, Clontech, Mountain View, CA, USA) administration [22], we show that TH fosters the expression of key mediators of the Warburg effect of cancer cells, namely, GLUT1, PKM2 and LDHA.