Varying levels of LGR5 expressions have been reported in 2D cultures of different human colorectal carcinoma cell lines, including the widely used Caco cells [44] in mouse primary colonic epithelial cell lines [45], and, at very low levels, in pig IPEC-J2 intestinal epithelial cell lines [46]. The gene discussed is LGR5; the disease is colorectal carcinoma.