Our findings validate the role of Ago2 as a key player in the antiviral response in Ae. aegypti during Semliki Forest virus (SFV; Togaviridae, Alphavirus) and Bunyamwera orthobunyavirus (BUNV; Bunyavirales, Peribunyaviridae), but not ZIKV, infection. This evidence concerns the gene AGO2 and infection.