miR-204-5p, with an effective role in male infertility, was presented as a possible regulator of TMPRSS2. Supplementary File 1 shows the list of all predicted miRNAs as regulators of ACE2 and TMPRSS2, the miRNAs associated with male infertility, and the extracted miRNAs from the predicted ones which were also associated with male infertility. This evidence concerns the gene TMPRSS2 and male infertility.