In the analysis of the global MS, significant differences were observed between healthy children and children with VSD in terms of MTHFR and AXIN1 genes (0.88 ± 1.71 vs. 3.32 ± 4.44, p = 0.001; 89.57 ± 42.52 vs. 58.74 ± 28.47, p = 0.012, respectively; Table 4). This evidence concerns the gene MTHFR and ventricular septal defect.