However, when the spheres were grown in the presence of concentrated supernatant from the broth culture of L. lactis(“empty” vector) or culture medium alone, as controls, they formed characteristic tumor packaging with much larger diameters (97.92–50.78 μm depending on the volume of the supernatant from the control bacteria, corresponding to the TRAIL-concentration range of 50–1000 ng/mL, or 134.89 μm, p < 0.001, respectively). Here, TNFSF10 is linked to neoplasm.