When short term (13 days) CII immunization experiments were performed, in pre-arthritis stage, we did not find differences in the fractions of total CD8+ (Figure 6A) or in CD8+ T-cells subpopulations in p110α−/−ΔT mice (Supplementary Materials Figure S2B), making a difference with CD4+ T cells (Figure 6); but when cell numbers in the lymph node were considered, we found a clear increase of CD8+ subpopulations in the animals bearing the genetic modification of p110α PI3K (Supplementary Materials Figure S2C), as was observed in CD4+ cells. This evidence concerns the gene CD4 and arthritic joint disease.