Treating macrophages with PANC-1 scrambled EVs significantly induced the upregulation of tumor-supportive genes such as IL-1β, TNFα, IL-6, and MMP9 as presented in Figure 4B. As TNFα gene was significantly upregulated by the oncogenic mutant p53 harboring EVs, we next took the medium for TNFα ELISA for protein secretion. The gene discussed is MMP9; the disease is neoplasm.