In H358 lung carcinoma cells, we used Tetracycline-controlled transcriptional activation (Tet-On inducible) cell system overexpressing several different p53 GOF mutants (V157F, R175H, R249S, and R273H), which simulates inactivated p53 compared with the WT form and an empty vector (ConVec) (described in detail here [22]). The gene discussed is TP53; the disease is lung carcinoma.