Studies from our lab also reported that silencing AEG-1 impedes proliferation and decreases invasive potential in colon cancer cell lines through interaction with Exostosin-1 (EXT-1), a well-known tumor suppressor of Hh signaling pathway, which plays a monumental role in chain elongation step of heparan sulfate biosynthesis, simultaneously upregulating it whilst AEG-1 was silenced [39]. This evidence concerns the gene MTDH and colonic neoplasm.