The effect of HCQ in inducing the apoptosis of HCC cells was assayed by annexin V/propidium iodide detection kit; as shown in Figure 2D, the treatment of HCQ on both the HCC cells resulted in a significant increase in the ratio of early and late apoptosis cells, while the percentage of viable cells reduced. The gene discussed is ANXA5; the disease is hepatocellular carcinoma.