In an attempt to investigate the role of TLR9 in HCC sorafenib resistance, publically available GEO microarray data (GSE94550) of Huh7 sorafenib resistance (n = 3) and sensitive cells (n = 3) were analyzed showing TLR9, ATG5/7/12, LC3B, and BECN1 were significantly overexpressed, whereas the oxidative stress marker (SOD1 enzyme) expression was reduced in HCC sorafenib-resistant Huh7 cells (Figure 1A). This evidence concerns the gene ATG5 and hepatocellular carcinoma.