After being cocultured with HCC-derived EVs for more than 30 days, the expression of stem cell-related proteins, such as OCT4, NANOG, SRY-box 2 (SOX2), P53, and CD133, in hepatocytes notably increased and these hepatic cells could still be subcultured compared with those not cocultured with HCC-derived EVs [80]. This evidence concerns the gene POU5F1 and hepatocellular carcinoma.