In conclusion, we identified 12 RBPs (RPS3, RBM22, EIF2S1, DHX8, RBM8A, UPF1, YBX1, SNRPE, SF3A1, U2AF1, SUPT6H, EIF3G) with strong tumor proliferative effects without any marked changes in expression in colorectal, pancreatic and esophageal cancer using the functional assessment of RBPs with an siRNA library focused on cancer growth change. This evidence concerns the gene U2AF1 and cancer.