From these hub gene sets, six key genes—YAP1, FOXA2, AMOTL2, TEAD2, SMAD4 and NF2—were selected for co-regulatory network construction as a result of their confirmed association through other databases with Hippo signaling pathway, cell proliferation/differentiation and NAFLD pathogenesis. The gene discussed is SMAD4; the disease is metabolic dysfunction-associated steatotic liver disease.