However, transmembrane TNFα (26 kd isoform) content, analyzed by Western blotting, was significantly increased in hyperammonemic rats (141 ± 10% of controls, p < 0.05), but bicuculline treatment decreased its content to control values (84 ± 10% of controls, p < 0.01, compared with hyperammonemia without bicuculline). Here, TNF is linked to Hyperammonemia.