The extracellular concentration of GABA was decreased by bicuculline in hyperammonemic rats (17 ± 7% nM, p < 0.05 compared with hyperammonemia without bicuculline) (F (3, 21) = 4.329, p < 0.05) (Figure 6e), surely due to a reversal of the increase of GAT-3 membrane expression, as GAT-1 remained decreased in hyperammonemic rats treated with bicuculline. Here, SLC6A1 is linked to Hyperammonemia.