Secreted cytokines (IFN-γ, TNF-α, IL-1β, IL-10, IL-12p70, KC/GRO, IL-6, IL-5, IL-4, and IL-2) in the bronchoalveolar lavage fluid (BALF) were quantified on day 6 post-infection following i.n. inoculation with LVS, LVSΔfptA, or LVSΔfptF, or mock infection with PBS using a 10-plex electrochemiluminescent detection method. Here, CALCA is linked to infection.