In Lewis lung carcinoma cells, the in vitro experiment on treatment with 10, 20 or 40 μM of lycopene caused increased IFNβ, IFNγ, IRF1, IRF7, CXCL9, CXCL10, pJAK and pSTAT3 mRNA expression while suppressed mRNA expression of DMNT3a, methylation levels of promoters (IRF1, IRF7) and PD-1 as induced by IFNγ via suppression in phosphorylation of Akt. The gene discussed is IFNG; the disease is Carcinoma, Lewis Lung.