In Lewis lung carcinoma cells, the in vitro experiment on treatment with 10, 20 or 40 μM of lycopene caused increased IFNβ, IFNγ, IRF1, IRF7, CXCL9, CXCL10, pJAK and pSTAT3 mRNA expression while suppressed mRNA expression of DMNT3a, methylation levels of promoters (IRF1, IRF7) and PD-1 as induced by IFNγ via suppression in phosphorylation of Akt. This evidence concerns the gene IRF1 and Carcinoma, Lewis Lung.