The transcriptomic data revealed a significant induction of HSR signature genes in both HNSCC spheroid types upon HT and HT + RT, i.e., the expression of some genes of the Hsp70 and Hsp40 families was substantially enhanced, such as HSPA6, HSPA1A, HSPA1B, HSPA1L, as well as DNAJA4, DNAJB1, and DNAJB4 (Figure 4a). This evidence concerns the gene HSPA1B and head and neck squamous cell carcinoma.