We validated the expression of 9 genes in these related pathways using real-time qRT-PCR and demonstrated that Cacna1c, Gabra5, Hcn4, Htr1b, Kcnb1, Scn2a, Bdnf, Creb, and Slc18a2 were increased in SS compared with the control. The gene discussed is HCN4; the disease is synovial sarcoma.