The results of this study showed that the CHK1 inhibitor V158411 decreased cell proliferation and induced DNA damage in the CRC cells HT29 and U2OS, assessed by nuclear staining for γ-H2AX, pCHK1 (S317), pCHK2 (T68), and pRPA32 (S4/S8). Here, CHEK1 is linked to colorectal carcinoma.