For the B16 melanoma cell line and the two human breast cancer cell lines, MDA-MB-231 and MCF-7, we found that hyperthermia alone had no significant effect on the expression of both immunosuppressive (PD-L1, PD-L2, HVEM and Gal-9) and immunostimulatory (ICOS-L, CD137-L, Ox40-L, CD27-L) checkpoint molecules and of EGFR. The gene discussed is TNFSF4; the disease is melanoma.