In this work, to further investigate the mechanisms underlying the neuroprotective action of Semax under the conditions of the tMCAO model, the expression of the pJNK, MMP-9, c-Fos, and pCREB proteins, which are actively involved in the pathogenesis of ischemic stroke, was analyzed by immunodetection in the subcortical structures and the frontoparietal cortex of the rat ipsilateral hemisphere. This evidence concerns the gene FOS and ischemic stroke.