In order to investigate the role of nucleophosmin and its interaction partner c-Myc in the response and development of acquired resistance to BRAFV600E inhibition by vemurafenib (PLX4032), we measured the expression levels of NPM1, c-Myc and their phosphorylated forms p-NPM1 (Thr199) and p-c-Myc (Ser62) in sensitive colon cancer cell lines HT-29 and RKO and their vemurafenib-resistant counterparts HT-29r and RKOr, respectively, after exposure to cytotoxic concentrations (IC50) of PLX4032 (Supplementary Table S5) for 24, 48 and 72 h. This evidence concerns the gene MYC and malignant colon neoplasm.