Baseline expression levels of selected protein targets were investigated by western blot in two BRAFV600E mutant colon cancer cell lines harboring WT KRAS (HT-29 and RKO), two WT BRAF cell lines carrying KRAS mutations (HCT 116 and SW480) and a double WT colon cancer cell line (Caco-2). This evidence concerns the gene KRAS and malignant colon neoplasm.