CD4 and neoplasm: Then, additional analyses demonstrated that the spleen subsets of CD4+ TNFR2+, CD4+ FOXP3+, and CD4+ TNFR2+ FOXP3+ T cells were all significantly down-regulated upon chemotherapy treatments (Figure 8(A1,B1)) and tended to correlate with higher tumor volumes (Figure 8(A2,B2,B3)); the subset of CD4+ TNFR2+ PD-1+ splenocytes showed a tendency toward down-regulation following chemotherapy, though not in a significant manner (Figure 8(C1,C2)).