In this study, Nestin expression was present mainly in the cytoplasm of tumor cells and showed an inverse relationship with the stromal/tumoral CD8+, CD4+ and FOXP3+ cell densities and CD8+/FOXP3+ ratios similar to other CSC markers studied (i.e., NANOG and SOX2), although the only significant association reached was with stromal FOXP3+ TILs. This evidence concerns the gene NANOG and neoplasm.