In fact, previous studies have reported that oxidative stress accelerated lipid peroxidation with the depletion of antioxidant enzymes, such as GSH and SOD, during the progression of NAFLD, and long-chain fatty acids (palmitate) induced oxidative stress and apoptotic responses in hepatic cells with steatosis [54,55]. This evidence concerns the gene SOD1 and metabolic dysfunction-associated steatotic liver disease.