We speculate that the m6A hypomethylation in these two key molecules (TLR4 and TIRAP) by MeRIP-seq and IGV visualization reduces the rapid inflammatory reaction of MAC-T treated with E. coli. The findings regarding m6A modification of these genes suggest that differential m6A modification somewhat enriches the pathogenic mechanism of E. coli infection of bovine mammary glands. Here, TLR4 is linked to escherichia coli infection.