TIRAP and escherichia coli infection: We speculate that the m6A hypomethylation in these two key molecules (TLR4 and TIRAP) by MeRIP-seq and IGV visualization reduces the rapid inflammatory reaction of MAC-T treated with E. coli. The findings regarding m6A modification of these genes suggest that differential m6A modification somewhat enriches the pathogenic mechanism of E. coli infection of bovine mammary glands.