To analyze the acid susceptibility and the NFκB and Nrf2/Keap1-pathway activation, we will attract attention to cellular viability due to inflammation and potential activation of the Nrf2/Keap1-pathway, resulting in resistance to chemotherapy in a Barrett’s esophagus in vitro model and an organotypic 3D-cultures system. Here, KEAP1 is linked to esophageal adenocarcinoma.