In an attempt to examine whether CB1 receptor signal might be involved in the upregulation of TFR2 implicated in hepatic iron overload [22], we analyzed the mRNA levels of TFR2 in HepG2, a human hepatoblastoma-derived cell line, and AML12, a mouse hepatocyte cell line after treatment with ACEA, a selective CB1 receptor agonist, in a time-dependent manner. This evidence concerns the gene TFR2 and hepatoblastoma.