To evaluate the expression patterns of genes involved in tumor-induced osteolysis with respect to α2β1 integrin, we performed qPCR and western blot analysis for PTHrP and Gli2 (Figure 5A,B) and TGFβrII and RUNX2 (Supplemental Figure S4) in MDA-Ctrl or MDA-OEα2 cells, and MDA-Parental or MDA-Bone cells. This evidence concerns the gene PTHLH and neoplasm.