In SW-48, Colo205 and HT-29 CRC cell lines, though Tribbles levels were not investigated, afatinib treatment (1 or 10 μM, 48 h) also reduced cell viability [95], representing an opportunity for further studies, in order to establish TRIB2 targeting as a potential strategy for treating colon cancer. Here, TRIB2 is linked to colonic neoplasm.