INSIG1 and metabolic dysfunction-associated steatotic liver disease: However, we believe that our results on the PNPLA3 variant, on the low expression of INSIG-1 and NPC1L1 representing a high intracellular cholesterol content and on the activation of HSC and fibrosis as factors associated with a particular attitude of the liver for the formation of Ld-MaS can be extrapolated to NAFLD patients.