Ten genes (namely, ADAMTS12, CCDC181, CD44, EPAS1, FILIP1L, KCTD8, NAALAD2, NEK9, PRKCB, and ZMIZ1) were selected for further validation in 151 PCa, 51 NPT, and 17 BPH samples (Table S2), based on the methylation differences in promoter regions according to prostate tissue histology (ADAMTS12, CCDC181, CD44, FILIP1L, KCTD8, NAALAD2, PRKCB, ZMIZ1) or BCR status (EPAS1, FILIP1L, NEK9, PRKCB, ZMIZ1). Here, KCTD8 is linked to benign prostatic hyperplasia.