The FFPE sections were stained in a sequential, multiplex, immunofluorescence staining protocol (Figure 6d) to enable visualization and quantification of the following six markers: GLUT1, HK2, HIF1α (glycolytic markers), CD68 (a macrophage marker), CD163 (a tumor-associated, or M2-associated marker), and DAPI (nuclear marker). This evidence concerns the gene HIF1A and neoplasm.