These regions were then projected in all sequential slides to perform additional CD3 immunohistochemistry for quantification of T-cell variability in the 3-dimensions of the tumor as well as IMC, mIF, WES, RNA-seq, TCR-seq, and A-FRET detection of the PD1-PDL1 interaction in situ (Figure 1B). Here, PDCD1 is linked to neoplasm.