WES also showed that the TMB in 3 patients (UH2, UH3, and UH6) was lower than 12 mutations/megabase pair (TCGA lower bound of CRC hypermutated phenotype23) despite negative MLH1 and PMS2 immunostaining and consistent with resistance to treatment (Supplementary Table 1). The gene discussed is MLH1; the disease is colorectal carcinoma.